Nuclear detection of cellular retinoic acid binding proteins I and II with new antibodies

被引：55

作者：

Gaub, MP ^{[1
]}

Lutz, Y ^{[1
]}

Ghyselinck, NB ^{[1
]}

Scheuer, I ^{[1
]}

Pfister, V ^{[1
]}

Chambon, P ^{[1
]}

Rochette-Egly, C ^{[1
]}

机构：

[1] CU Strasbourg, IGBMC, ULP, Coll France,INSERM,CNRS, F-67404 Illkirch, France

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1998年 / 46卷 / 10期

关键词：

cellular retinoic acid binding proteins; antibodies; immunodetection; nuclear localization;

D O I：

10.1177/002215549804601002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Apart from the retinoic acid nuclear receptor family, there are two low molecular weight (15 kD) cellular retinoic acid binding proteins, named CRABPI and II. Mouse monoclonal and rabbit polyclonal antibodies were raised against these proteins by using as antigens either synthetic peptides corresponding to amino acid sequences unique to CRABPI or CRABPII, or purified CRABP proteins expressed in E. coli. Antibodies specific for mouse and/or human CRABPI and CRABPII were obtained and characterized by immunocytochemistry and immunoblotting. They allowed the detection not only of CRABPI but also of CRABPII in both nuclear and cytosolic extracts from transfected COS-l cells, mouse embryos, and various cell lines.

引用

页码：1103 / 1111

页数：9

共 36 条

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