Functional analysis of a mitochondrial phosphopantetheinyl transferase (PPTase) gene pptB in Aspergillus fumigatus

被引：23

作者：

Allen, Gethin ^{[1
]}

Bromley, Mike ^{[2
]}

Kaye, Sarah J. ^{[2
]}

Keszenman-Pereyra, David ^{[1
]}

Zucchi, Tiago D. ^{[1
]}

Price, Jaqueline ^{[1
]}

Birch, Mike ^{[2
]}

Oliver, Jason D. ^{[2
]}

Turner, Geoffrey ^{[1
]}

机构：

[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire, England

[2] F2G Ltd, Manchester M30 0BH, Lancs, England

来源：

FUNGAL GENETICS AND BIOLOGY | 2011年 / 48卷 / 04期

关键词：

Aspergillus fumigatus; Essential genes; Phosphopantetheinyl transferase; Siderophore; Respiration; PptA; PptB; NpgA; ACYL-CARRIER PROTEIN; 4'-PHOSPHOPANTETHEINYL TRANSFERASE; SACCHAROMYCES-CEREVISIAE; SECONDARY METABOLISM; LYSINE BIOSYNTHESIS; NIDULANS; SYNTHASE; INHIBITORS; IDENTIFICATION; PATHOGENICITY;

D O I：

10.1016/j.fgb.2010.12.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The mitochondrial phosphopantetheinyl transferase gene pptB of the opportunistic pathogen Aspergillus fumigatus has been identified and characterised. Unlike pptA, which is required for lysine biosynthesis, secondary metabolism, and iron assimilation, pptB is essential for viability. PptB is located in the mitochondria. In vitro expression of pptA and pptB has shown that PptB is specific for the mitochondrial acyl carrier protein AcpA. (c) 2010 Elsevier Inc. All rights reserved.

引用

页码：456 / 464

页数：9

共 37 条

[1] DEVELOPMENT OF A HIGH-FREQUENCY TRANSFORMING VECTOR FOR ASPERGILLUS-NIDULANS [J].

BALLANCE, DJ ;

TURNER, G .

GENE, 1985, 36 (03) :321-331

[2] Mitochondrial acyl carrier protein is involved in lipoic acid synthesis in Saccharomyces cerevisiae [J].

Brody, S ;

Oh, CK ;

Hoja, U ;

Schweizer, E .

FEBS LETTERS, 1997, 408 (02) :217-220

[3] Mechanism and substrate recognition of human holo ACP synthase [J].

Bunkoczi, Gabor ;

Pasta, Saloni ;

Joshi, Anil ;

Wu, Xiaoqiu ;

Kavanagh, Kathryn L. ;

Smith, Stuart ;

Oppermann, Udo .

CHEMISTRY & BIOLOGY, 2007, 14 (11) :1243-1253

[4] The Transposon impala Is Activated by Low Temperatures: Use of a Controlled Transposition System To Identify Genes Critical for Viability of Aspergillus fumigatus [J].

Carr, Paul D. ;

Tuckwell, Danny ;

Hey, Peter M. ;

Simon, Laurence ;

d'Enfert, Christophe ;

Birch, Mike ;

Oliver, Jason D. ;

Bromley, Michael J. .

EUKARYOTIC CELL, 2010, 9 (03) :438-448

[5] Computational method to predict mitochondrially imported proteins and their targeting sequences [J].

Claros, MG ;

Vincens, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 241 (03) :779-786

[6] Acyl-carrier protein-phosphopantetheinyltransferase partnerships in fungal fatty acid synthases [J].

Crawford, Jason M. ;

Vagstad, Anna L. ;

Ehrlich, Kenneth C. ;

Udwary, Daniel W. ;

Townsend, Craig A. .

CHEMBIOCHEM, 2008, 9 (10) :1559-1563

[7] Therapy for fungal diseases: opportunities and priorities [J].

Denning, David W. ;

Hope, William W. .

TRENDS IN MICROBIOLOGY, 2010, 18 (05) :195-204

[8] Lysine biosynthesis in Saccharomyces cerevisiae:: Mechanism of α-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5 [J].

Ehmann, DE ;

Gehring, AM ;

Walsh, CT .

BIOCHEMISTRY, 1999, 38 (19) :6171-6177

[9] The akuBKU80 mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus [J].

Ferreira, MED ;

Kress, MRVZ ;

Savoldi, M ;

Goldman, MHS ;

Härtl, A ;

Heinekamp, T ;

Brakhage, AA ;

Goldman, GH .

EUKARYOTIC CELL, 2006, 5 (01) :207-211

[10] A novel function of yeast fatty acid synthase -: Subunit α is capable of self-pantetheinylation [J].

Fichtlscherer, F ;

Wellein, C ;

Mittag, M ;

Schweizer, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (09) :2666-2671

← 1 2 3 4 →