Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16

被引：54

作者：

Dinu, Diana

Nechifor, Marina Tamara

Stoian, Gheorghe

Costache, Marieta

Dinischiotu, Anca

机构：

[1] Univ Bucharest, Fac Biol, Dept Biochem, Bucharest 050095, Romania

[2] Univ Bucharest, Fac Biol, Dept Anim Biol, Bucharest 050095, Romania

来源：

JOURNAL OF BIOTECHNOLOGY | 2007年 / 131卷 / 02期

关键词：

Aspergillus niger; acidic pectate lyases; homogenous pectinases characterization; pectin methylesterase; polygalacturonase; purification;

D O I：

10.1016/j.jbiotec.2007.06.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were analyzed. The purified enzymes were classified with respect to their biochemical properties and substrate specificity. Among these proteins, one revealed polygalacturonase activity, another appeared to be a pectin methylesterase and three were identified as pectate lyases. The capacity of the fungus A. niger to produce pectate lyases with optimum pH in acidic domain was reported for the first time. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：128 / 137

页数：10

共 42 条

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