Enzyme-catalyzed reaction of OPD-H2O2-HRP voltammetric enzyme-linked immunoassay system

被引：18

作者：

Jiao, K ^{[1
]}

Sun, G ^{[1
]}

Zhang, SS ^{[1
]}

机构：

[1] Qingdao Inst Chem Technol, Dept Appl Chem, Qingdao 266042, Peoples R China

来源：

SCIENCE IN CHINA SERIES B-CHEMISTRY | 1998年 / 41卷 / 04期

关键词：

o-phenylenediamine; voltammetry; immunoassay; enzyme-catalyzing; horseradish peroxidase;

D O I：

10.1007/BF02877812

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Enzyme-catalyzed reaction of o-phenylenediamine (OPD)-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay system has been studied in detail with electrochemical analysis, high performance liquid chromatography (HPLC), ultraviolet/visible (UV/Vis) spectroscopy, infrared (IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The pure product of H2O2 oxidizing OPD catalyzed by HRP was prepared with chemical method. The experimental results of voltammetry and HPLC indicate that only one product of enzyme-catalyzed reaction has been obtained under the selected enzyme-catalyzed reaction conditions. Identifications by UV/Vis spectrum, IR spectrum and C-13 NMR spectrum show that the product is 2, 3-diaminophenazine, The processes of the enzyme-catalyzed reaction and the electroreduction of the product of the enzyme-catalyzed reaction are described.

引用

页码：345 / 352

页数：8

共 14 条

[1]

EVERSE J, 1991, PEROXIDASE CHEM BIOL, V2, P5

[2]

Furnica M., 1978, B I POLITECH IASI, V24, P35

[3]

GALLATI H, 1982, J CLIN CHEM CLIN BIO, V20, P221

[4]

GAO XX, 1986, INTRO ELECTROCHEMICA

[5]

Griess P., 1871, J PRAKT CHEM, V3, P143

[6]

HE YN, 1997, CHINESE J ANAL CHEM, V25, P30

[7]

Jiao K, 1997, ACTA CHIM SINICA, V55, P1121

[8]

JIAO K, 1995, CHINESE J ANAL CHEM, V23, P1211

[9]

JIAO K, 1996, SCI CHINA SER B, V39, P137

[10]

Knoevnagel E., 1914, J PRAKT CHEM, V89, P25

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