Separation of phenolic aldehydes, ketones and acids from lignin degradation by capillary zone electrophoresis

被引:45
作者
Maman, O
Marseille, F
Guillet, B
Disnar, JR
Morin, P
机构
[1] UNIV ORLEANS,LAB GEOCHIM ORGAN,URA CNRS 724,F-45067 ORLEANS,FRANCE
[2] UNIV ORLEANS,INST CHIM ORGAN & ANAL,CNRS URA 499,F-45067 ORLEANS,FRANCE
关键词
ketones; aldehydes; phenolic acids; phenolic compounds; lignin;
D O I
10.1016/S0021-9673(96)00583-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary zone electrophoresis (CZE) was investigated for the separation of eleven phenolic aldehydes, ketones and acids which are main components of lignin. The influence of the buffer pH, electrolyte composition, acetonitrile amount, temperature and voltage has been investigated to determine the best separation conditions. Using optimized buffer [55 mM borate-phosphate (pH 9.1) /acetonitrile (91.5-8.5, v/v)], efficient separations occur in less than 15 min with a good repeatability of the electropherograms, giving a relative standard deviation of each peak of less than 1%. The method was applied to the analysis of phenolic compounds produced by the CuO oxidation of foliar organs of gymnosperm and angiosperm trees. Results are in agreement with data obtained by other chromatographic methods.
引用
收藏
页码:89 / 97
页数:9
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