Human antibodies from immunized donors are protective against anthrax toxin in vivo

被引：75

作者：

Wild, MA

Xin, H

Maruyama, T

Nolan, MJ

Calveley, PM

Malone, JD

Wallace, MR

Bowdish, KS

机构：

[1] Alex Antibody Technol Inc, San Diego, CA 92121 USA

[2] Natl Naval Med Res Inst, San Diego, CA 92134 USA

来源：

NATURE BIOTECHNOLOGY | 2003年 / 21卷 / 11期

关键词：

D O I：

10.1038/nbt891

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A panel of Fabs that neutralize anthrax toxin in vitro was selected from libraries generated from human donors vaccinated against anthrax. At least two of these antibodies protect rats from anthrax intoxication in vivo. Fabs 83K7C and 63L1D bind with subnanomolar affinity to protective antigen (PA) 63, and Fab 63L1D neutralizes toxin substoichiometrically, inhibits lethal factor (LF) interaction with PA63 and binds to a conformational epitope formed by PA63.

引用

页码：1305 / 1306

页数：2

共 7 条

[1] ANTHRAX TOXIN PROTECTIVE ANTIGEN IS ACTIVATED BY A CELL-SURFACE PROTEASE WITH THE SEQUENCE SPECIFICITY AND CATALYTIC PROPERTIES OF FURIN [J].