Quantitative automated particle-enhanced immunonephelometric assay for the routinary measurement of human cystatin C

被引:65
作者
Mussap, M
Ruzzante, N
Varagnolo, M
Plebani, M
机构
[1] Univ Hosp Padova, Dept Lab Med, Padua, Italy
[2] Castelfranco V To, Ctr Biomed Res, Veneto, Italy
关键词
cystatin C; glomerular filtration rate; latex immunoassay; nephelometry; renal function;
D O I
10.1515/CCLM.1998.151
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Human cystatin C is a low molecular mass protein of 13359 Dalton recently proposed as a new very sensitive marker of changes in glomerular filtration rate. Serum cystatin C concentration correlates negatively with glomerular filtration rate as well as or better than creatinine. We evaluated a recently introduced automated nephelometric immunoassay for cystatin C in serum or EDTA-plasma samples on the Behring Nephelometer System. The assay consists of incubating the 100-fold diluted sample for 6 minutes with latex particles covalently coated with anti-human cystatin C antibodies, and then quantifying the change of light-scatter produced. Method reproducibility is satisfactory, the intra- and inter-assay coefficients of variation ranging from 1.58 % to 3.77 % and from 5.6 % to 11.47 % respectively. Rheumatoid factor (less than or equal to 1116 IU/ml), bilirubin (less than or equal to 418 mu mmol/l), triglycerides (10.47 mmol/l), and haemoglobin (12 g/l) do not significantly interfere in the assay. No significant difference was found in cystatin C concentration between serum and EDTA-plasma samples. Cystatin C is stable in serum samples stored under different conditions up to one month. This method correlates well (mean difference = -0.536 +/- 0.307 mg/l) with another commercially available particle-enhanced turbidimetric immunoassay. Cystatin C offers better clinical sensitivity than creatinine for discriminating patients with normal renal function and those with mild-to-moderate reduction in renal function. This method is suitable for routine cystatin C measurement, including emergencies.
引用
收藏
页码:859 / 865
页数:7
相关论文
共 35 条
[1]   STRUCTURE AND EXPRESSION OF THE HUMAN CYSTATIN-C GENE [J].
ABRAHAMSON, M ;
OLAFSSON, I ;
PALSDOTTIR, A ;
ULVSBACK, M ;
LUNDWALL, A ;
JENSSON, O ;
GRUBB, A .
BIOCHEMICAL JOURNAL, 1990, 268 (02) :287-294
[2]  
ABRAHAMSON M, 1986, J BIOL CHEM, V261, P1282
[3]  
*BANGS LAB, 1987, UN LAT PART, P25
[4]   NOMENCLATURE AND CLASSIFICATION OF THE PROTEINS HOMOLOGOUS WITH THE CYSTEINE-PROTEINASE INHIBITOR CHICKEN CYSTATIN [J].
BARRETT, AJ ;
FRITZ, H ;
GRUBB, A ;
ISEMURA, S ;
JARVINEN, M ;
KATUNUMA, N ;
MACHLEIDT, W ;
MULLERESTERL, W ;
SASAKI, M ;
TURK, V .
BIOCHEMICAL JOURNAL, 1986, 236 (01) :312-312
[5]   MICROGLOBULINAEMIA IN RENAL FAILURE [J].
BERNIER, GM ;
COHEN, RJ ;
CONRAD, ME .
NATURE, 1968, 218 (5141) :598-&
[6]  
Bertelli M., 1997, MED SURG PED, V19, P325
[7]   STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT [J].
BLAND, JM ;
ALTMAN, DG .
LANCET, 1986, 1 (8476) :307-310
[8]   THE 2.0 A X-RAY CRYSTAL-STRUCTURE OF CHICKEN EGG-WHITE CYSTATIN AND ITS POSSIBLE MODE OF INTERACTION WITH CYSTEINE PROTEINASES [J].
BODE, W ;
ENGH, R ;
MUSIL, D ;
THIELE, U ;
HUBER, R ;
KARSHIKOV, A ;
BRZIN, J ;
KOS, J ;
TURK, V .
EMBO JOURNAL, 1988, 7 (08) :2593-2599
[9]   Cystatin C - A new marker of glomerular filtration rate in children independent of age and height [J].
Bokenkamp, A ;
Domanetzki, M ;
Zinck, R ;
Schumann, G ;
Byrd, D ;
Brodehl, J .
PEDIATRICS, 1998, 101 (05) :875-881
[10]   Reference values for cystatin C serum concentrations in children [J].
Bökenkamp, A ;
Domanetzki, M ;
Zinck, R ;
Schumann, G ;
Brodehl, J .
PEDIATRIC NEPHROLOGY, 1998, 12 (02) :125-129