Phenotyping two tomato genotypes with different nitrogen use efficiency

被引:64
作者
Abenavoli, Maria Rosa [1 ]
Longo, Caterina [1 ]
Lupini, Antonio [1 ]
Miller, Anthony J. [2 ]
Araniti, Fabrizio [1 ]
Mercati, Francesco [3 ]
Princi, Maria P. [1 ]
Sunseri, Francesco [1 ]
机构
[1] Univ Mediterranea Reggio Calabria, Dipartimento AGR, I-89124 Reggio Di Calabria, Italy
[2] John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England
[3] Natl Res Council Italy CNR, Inst Biosci & Bioresources IBBR, Corso Calatafimi 414, I-90129 Palermo, Italy
关键词
Nitrate influx; Nitrate reductase; Nitrate transporter genes; Nitrogen Uptake Efficiency - NUpE; Nitrogen Utilization Efficiency - NUtE; Nitrate uptake; Tomato (Solanum lycopersicon L.); AFFINITY NITRATE TRANSPORTERS; UPTAKE SYSTEM; ARABIDOPSIS; ASSIMILATION; EXPRESSION; PLANTS; TRANSLOCATION; ACQUISITION; REDUCTASE; PROTEIN;
D O I
10.1016/j.plaphy.2016.04.021
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nitrogen (N) supply usually limits crop production and optimizing N-use efficiency (NUE) to minimize fertilizer loss is important. NUE is a complex trait that can be dissected into crop N uptake from the soil (NUpE) and N utilization (NUtE). We compared NUE in 14 genotypes of three week old tomatoes grown in sand or hydroponic culture supplied with nitrate (NOT). Culture method influenced measured NUE for some cultivars, but Regina Ostuni (RO) and UC82 were consistently identified as high and low NUE genotypes. To identify why these genotypes had contrasting NUE some traits were compared growing under 0.1 and 5 mM NOT supply. UC82 showed greater root 15No(3)(-) influx at low and high supply, and stronger SINRT2.1/NAR2.1 transporter expression under low supply when compared with RO. Conversely, RO showed a higher total root length and thickness compared to UC82. Compared with UC82, RO showed higher shoot SlNRT2.3 expression and NO3- storage at high supply, but similar NOT reductase activity. After N-starvation, root cell electrical potentials of RO were significantly more negative than UC82, but nitrate elicited similar responses in both root types. Overall for UC82 and RO, NUtE may play a greater role than NUpE for improved NUE. (C) 2016 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:21 / 32
页数:12
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