A compact functional quantum dot-DNA conjugate: Preparation, hybridization, and specific label-free DNA detection

被引：120

作者：

Zhou, Dejian ^{[1
]}

Ying, Liming ^{[2
]}

Hong, Xin ^{[3
]}

Hall, Elizabeth A. ^{[3
]}

Abell, Chris ^{[1
]}

Klenerman, David ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[2] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London SW7 2AZ, England

[3] Univ Cambridge, Inst Biotechnol, Cambridge CB2 1QT, England

来源：

LANGMUIR | 2008年 / 24卷 / 05期

关键词：

D O I：

10.1021/la703583u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this letter, we report the preparation of a compact, functional quantum dot (QD)-DNA conjugate, where the capturing target DNA is directly and covalently coupled to the QD surface. This enables control of the separation distance between the QD donor and dye acceptor to within the range of the Forster radius. Moreover, a tri(ethylene glycol) linker is introduced to the QD surface coating to effectively eliminate the strong, nonspecific adsorption of DNA on the QD surface. As a result, this QD-DNA conjugate hybridizes specifically to its complementary DNA with a hybridization rate constant comparable to that of free DNAs in solution. We show this system is capable of specific detection of nanomolar unlabeled complimentary DNA at low DNA probe/QD copy numbers via a "signal-on" fluorescence resonance energy transfer (FRET) response.

引用

页码：1659 / 1664

页数：6

共 39 条

[1] Adsorption and hybridization of oligonucleotides on mercaptoacetic acid-capped CdSe/ZnS quantum dots and quantum dot-oligonucleotide conjugates [J].

Algar, W. Russ ;

Krull, Ulrich J. .

LANGMUIR, 2006, 22 (26) :11346-11352

[2] The use of nanocrystals in biological detection [J].

Alivisatos, P .

NATURE BIOTECHNOLOGY, 2004, 22 (01) :47-52

[3] Quantum dot-conjugated hybridization probes for preliminary screening of siRNA sequences [J].

Bakalova, R ;

Zhelev, Z ;

Ohba, H ;

Baba, Y .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (32) :11328-11335

[4] Aptamers come of age - at last [J].

Bunka, David H. J. ;

Stockley, Peter G. .

NATURE REVIEWS MICROBIOLOGY, 2006, 4 (08) :588-596

[5] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[6] Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors [J].

Clapp, AR ;

Medintz, IL ;

Mauro, JM ;

Fisher, BR ;

Bawendi, MG ;

Mattoussi, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) :301-310

[7] Forster resonance energy transfer investigations using quantum-dot fluorophores [J].

Clapp, AR ;

Medintz, IL ;

Mattoussi, H .

CHEMPHYSCHEM, 2006, 7 (01) :47-57

[8] Single-molecule measurements of gold-quenched quantum dots [J].

Gueroui, Z ;

Libchaber, A .

PHYSICAL REVIEW LETTERS, 2004, 93 (16) :166108-1

[9] Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules [J].

Han, MY ;

Gao, XH ;

Su, JZ ;

Nie, S .

NATURE BIOTECHNOLOGY, 2001, 19 (07) :631-635

[10] Quadruplex-to-duplex transition of G-rich oligonucleotides probed by cationic water-soluble conjugated polyelectrolytes [J].

He, Fang ;

Tang, Yanli ;

Yu, Minghui ;

Feng, Fude ;

An, Lingling ;

Sun, Huan ;

Wang, Shu ;

Li, Yuliang ;

Zhu, Daoben ;

Bazan, Guillermo C. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (21) :6764-6765

← 1 2 3 4 →