Trichinella spiralis: Characterization of phage-displayed specific epitopes and their protective immunity in BALB/c mice

被引：27

作者：

Gu, Yuan ^{[1
]}

Li, Jie ^{[2
]}

Zhu, Xinping ^{[1
]}

Yang, Jing ^{[1
]}

Li, Qiang ^{[1
]}

Liu, Zhiying ^{[1
]}

Yu, Shun ^{[3
]}

Li, Yaohua ^{[3
]}

机构：

[1] Capital Med Univ, Sch Basic Med Sci, Dept Parasitol, Beijing 100069, Peoples R China

[2] Beijing Ctr Dis Prevent & Control, Beijing 100013, Peoples R China

[3] Capital Med Univ, Xuanwu Hosp, Beijing Inst Geriatr, Beijing 100053, Peoples R China

来源：

EXPERIMENTAL PARASITOLOGY | 2008年 / 118卷 / 01期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1016/j.exppara.2007.06.014

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Trichinellosis is a global zoonosis mainly caused by Trichinella spiralis. We have previously reported that a novel Ts87 gene from the cDNA library of adult T. spiralis was cloned and expressed in a prokaryotic expression system. Vaccination with recombinant Ts87 protein (rTs87) induced a muscle larvae burden reduction in BALB/c mice by 29% in response to T. spiralis infection. In the present study, we screened a random phage-displayed peptide library using monoclonal antibody 5A3 which recognized Ts87 protein. Four positive phage clones were selected to subcutaneously immunize BALB/c mice without adjuvant. Two phage clones could effectively stimulate specific antibodies against rTs87. Mice vaccinated with these two combined phage clones showed a 28.7% worm burden reduction as compared to the control group. Therefore, the identified phage clones displayed peptides representing specific epitopes of Ts87 protein and could be considered as potential vaccine candidates for T. spiralis. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：66 / 74

页数：9

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