Transport of apically but aot basolaterally internalized ricin to the Golgi apparatus is stimulated by 8-Br-cAMP in MDCK cells

被引：12

作者：

Llorente, A

van Deurs, B

Sandvig, K ^{[1
]}

机构：

[1] Norwegian Radium Hosp, Inst Canc Res, N-0310 Oslo, Norway

[2] Univ Copenhagen, Panum Inst, Dept Med Anat, Struct Cell Biol Unit, DK-2200 Copenhagen N, Denmark

来源：

FEBS LETTERS | 1998年 / 431卷 / 02期

关键词：

ricin; membrane traffic endosome; Golgi apparatus; cAMP; MDCK cell;

D O I：

10.1016/S0014-5793(98)00754-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant toxin ricin has to be transported to the Golgi apparatus after endocytosis to exert its toxic effect. In this study we show that transport of apically endocytosed ricin to the Golgi apparatus is stimulated by 8-Br-cAMP in polarized MDCK cells, This stimulation is counteracted by the PKA inhibitor H-89, In contrast, there is no increase in the transport to the Golgi apparatus of ricin internalized from the basolateral membrane. These results suggest that protein kinase A selectively regulates endosome to Golgi transport in these cells. (C) 1998 Federation of European Biochemical Societies.

引用

页码：200 / 204

页数：5

共 31 条

[1] An endosomal beta COP is involved in the pH-dependent formation of transport vesicles destined for late endosomes [J].