Acid-Degradable Cationic Dextran Particles for the Delivery of siRNA Therapeutics

被引：136

作者：

Cohen, Jessica L. ^{[1
]}

Schubert, Stephanie ^{[1
]}

Wich, Peter R. ^{[1
]}

Cui, Lina ^{[1
]}

Cohen, Joel A. ^{[1
]}

Mynar, Justin L. ^{[1
]}

Frechet, Jean M. J. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Coll Chem, Berkeley, CA 94720 USA

来源：

BIOCONJUGATE CHEMISTRY | 2011年 / 22卷 / 06期

基金：

美国国家卫生研究院;

关键词：

RNA INTERFERENCE; GENE DELIVERY; IN-VITRO; ADENOASSOCIATED VIRUS; ACETALATED DEXTRAN; MICROPARTICLES; VECTORS; POLYMERS; DESIGN; NANOPARTICLES;

D O I：

10.1021/bc100542r

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a new acid-sensitive, biocompatible, and biodegradable microparticulate delivery system, spermine modified acetalated-dextran (Spermine-Ac-DEX), which can be used to efficiently encapsulate siRNA. These particles demonstrated efficient gene knockdown in HeLa-luc cells with minimal toxicity. This knockdown was comparable to that obtained using Lipofectamine, a commercially available transfection reagent generally limited to in vitro use due to its high toxicity.

引用

页码：1056 / 1065

页数：10

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