A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes

被引：23

作者：

Ren, YL ^{[1
]}

Xing, XH ^{[1
]}

Zhang, C ^{[1
]}

Gou, ZX ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem Engn, Beijing 100084, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 14期

关键词：

Enterobacter aerogenes; hydrogen evolution; hydrogen uptake; hydrogenase activity; surfactant;

D O I：

10.1007/s10529-005-8106-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Assay of hydrogenase activity pertaining to H-2 production needs anaerobic conditions. To establish a simplified method for assay of hydrogenase activities by using intact cells of Enterobater aerogenes, different chemicals capable of enhancing the cell-wall permeability to electron mediators were examined. As a result, Triton X-100 and CTAB were found to be appropriate for H-2 uptake and evolution activities of the intact cells, respectively. This method enabled H-2 uptake and evolution activities of the intact cells to be easily detected. This is also the first report of the presence of H-2 uptake hydrogenase activity in E. aerogenes.

引用

页码：1029 / 1033

页数：5

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