Taxonomic and Functional Metagenomic Profiling of the Microbial Community in the Anoxic Sediment of a Sub-saline Shallow Lake (Laguna de Carrizo, Central Spain)

被引：49

作者：

Ferrer, Manuel ^{[1
]}

Guazzaroni, Maria-Eugenia ^{[1
]}

Richter, Michael ^{[2
]}

Garcia-Salamanca, Adela ^{[3
]}

Yarza, Pablo ^{[2
]}

Suarez-Suarez, Ana ^{[2
]}

Solano, Jennifer ^{[4
]}

Alcaide, Maria ^{[1
]}

van Dillewijn, Pieter ^{[3
]}

Molina-Henares, Maria Antonia ^{[3
]}

Lopez-Cortes, Nieves ^{[1
]}

Al-Ramahi, Yamal ^{[1
]}

Guerrero, Carmen ^{[5
]}

Acosta, Alejandro ^{[3
]}

de Eugenio, Laura I. ^{[6
]}

Martinez, Virginia ^{[6
]}

Marques, Silvia ^{[3
]}

Rojo, Fernando ^{[7
]}

Santero, Eduardo ^{[8
]}

Genilloud, Olga ^{[9
]}

Perez-Perez, Julian ^{[10
]}

Rossello-Mora, Ramon ^{[2
]}

Ramos, Juan Luis ^{[3
]}

机构：

[1] CSIC, Inst Catalysis, E-28049 Madrid, Spain

[2] IMEDEA CSIC UIB, Esporles 07190, Spain

[3] CSIC, Estn Expt Zaidin, E-18008 Granada, Spain

[4] Bio Illiberis R&D, Granada 18100, Spain

[5] Univ Autonoma Madrid, Fac Ciencias, E-28049 Madrid, Spain

[6] CSIC, Ctr Invest Biol, E-28049 Madrid, Spain

[7] CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

[8] Univ Pablo Olavide, Seville 41013, Spain

[9] Fdn Medina, Granada 18100, Spain

[10] Secugen, Madrid 28049, Spain

来源：

MICROBIAL ECOLOGY | 2011年 / 62卷 / 04期

关键词：

FRESH-WATER LAKE; BACTERIAL COMMUNITY; ARCHAEAL COMMUNITY; EUTROPHIC LAKE; PROKARYOTIC DIVERSITY; MARINE-SEDIMENTS; MEROMICTIC LAKE; SKAN BAY; SEQUENCE; MATS;

D O I：

10.1007/s00248-011-9903-y

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

The phylogenetic and functional structure of the microbial community residing in a Ca2+-rich anoxic sediment of a sub-saline shallow lake (Laguna de Carrizo, initially operated as a gypsum (CaSO4 x 2 H2O) mine) was estimated by analyzing the diversity of 16S rRNA amplicons and a 3.1 Mb of consensus metagenome sequence. The lake has about half the salinity of seawater and possesses an unusual relative concentration of ions, with Ca2+ and SO (4) (2-) being dominant. The 16S rRNA sequences revealed a diverse community with about 22% of the bacterial rRNAs being less than 94.5% similar to any rRNA currently deposited in GenBank. In addition to this, about 79% of the archaeal rRNA genes were mostly related to uncultured Euryarchaeota of the CCA47 group, which are often associated with marine and oxygen-depleted sites. Sequence analysis of assembled genes revealed that 23% of the open reading frames of the metagenome library had no hits in the database. Among annotated genes, functions related to (thio) sulfate and (thio) sulfonate-reduction and iron-oxidation, sulfur-oxidation, denitrification, synthrophism, and phototrophic sulfur metabolism were found as predominant. Phylogenetic and biochemical analyses indicate that the inherent physical-chemical characteristics of this habitat coupled with adaptation to anthropogenic activities have resulted in a highly efficient community for the assimilation of polysulfides, sulfoxides, and organosulfonates together with nitro-, nitrile-, and cyanide-substituted compounds. We discuss that the relevant microbial composition and metabolic capacities at Laguna de Carrizo, likely developed as an adaptation to thrive in the presence of moderate salinity conditions and potential toxic bio-molecules, in contrast with the properties of previously known anoxic sediments of shallow lakes.

引用

页码：824 / 837

页数：14

共 87 条

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