Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-styrene) hydrogel

被引：31

作者：

Basri, M ^{[1
]}

Harun, A ^{[1
]}

Ahmad, MB ^{[1
]}

Razak, CNA ^{[1
]}

Salleh, AB ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fak Sains & Pengajian Alam Sekitar, Serdang 43400, Malaysia

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2001年 / 82卷 / 06期

关键词：

lipase; hydrogels; polymerization; esterification; activation; stabilization;

D O I：

10.1002/app.1977

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Lipase from Candida rugosa was immobilized by entrapment while polymerizing a poly(N-vinyl-2-pyrrolidone-co-styrene) [poly(VP-co-ST)] hydrogel using ethylene dimethacrylate (EDMA) as the crosslinking agent. The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. The activities of the immobilized enzymes and the leaching ability of the enzyme from the support with respect to the different compositions of the hydrogels were investigated. The thermal, solvent, and storage stability of the immobilized lipases were also determined. The activities were relatively higher when the percent compositions of VP(%): ST(%) were 50:50 and 30:70. The lipase immobilized on VP(%):ST(%) 50:50 showed the highest thermal stability, while lipase immobilized on VP(%):ST(%) 30:70 exhibited the highest solvent stability. (C) 2001 John Wiley & Sons, Inc.

引用

页码：1404 / 1409

页数：6

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