Dynamics of Responses in Compatible Potato - Potato virus Y Interaction Are Modulated by Salicylic Acid

被引：62

作者：

Baebler, Spela ^{[1
]}

Stare, Katja ^{[1
]}

Kovac, Maja ^{[1
]}

Blejec, Andrej ^{[1
]}

Prezelj, Nina ^{[1
]}

Stare, Tjasa ^{[1
]}

Kogovsek, Polona ^{[1
]}

Pompe-Novak, Marusa ^{[1
]}

Rosahl, Sabine ^{[2
]}

Ravnikar, Maja ^{[1
]}

Gruden, Kristina ^{[1
]}

机构：

[1] Natl Inst Biol, Dept Biotechnol & Syst Biol, Ljubljana, Slovenia

[2] Leibniz Inst Plant Biochem, Dept Stress & Dev Biol, Halle, Saale, Germany

来源：

PLOS ONE | 2011年 / 6卷 / 12期

关键词：

GENE-EXPRESSION; DEFENSE-MECHANISMS; SOLANUM-TUBEROSUM; NTN INFECTION; REAL-TIME; PLANTS; RESISTANCE; CARBOHYDRATE; LEAVES; GENOTYPES;

D O I：

10.1371/journal.pone.0029009

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To investigate the dynamics of the potato - Potato virus Y (PVY) compatible interaction in relation to salicylic acid - controlled pathways we performed experiments using non-transgenic potato cv. Desiree, transgenic NahG-Desiree, cv. Igor and PVYNTN, the most aggressive strain of PVY. The importance of salicylic acid in viral multiplication and symptom development was confirmed by pronounced symptom development in NahG-Desiree, depleted in salicylic acid, and reversion of the effect after spraying with 2,6-dichloroisonicotinic acid (a salicylic acid - analogue). We have employed quantitative PCR for monitoring virus multiplication, as well as plant responses through expression of selected marker genes of photosynthetic activity, carbohydrate metabolism and the defence response. Viral multiplication was the slowest in inoculated potato of cv. Desiree, the only asymptomatic genotype in the study. The intensity of defence-related gene expression was much stronger in both sensitive genotypes (NahG-Desiree and cv. Igor) at the site of inoculation than in asymptomatic plants (cv. Desiree). Photosynthesis and carbohydrate metabolism gene expression differed between the symptomatic and asymptomatic phenotypes. The differential gene expression pattern of the two sensitive genotypes indicates that the outcome of the interaction does not rely simply on one regulatory component, but similar phenotypical features can result from distinct responses at the molecular level.

引用

页数：12

共 47 条

[1] Salicylic acid-mediated and RNA-silencing defense mechanisms cooperate in the restriction of systemic spread of plum pox virus in tobacco [J].

Alamillo, Josefa M. ;

Saenz, Pilar ;

Garcia, Juan Antonio .

PLANT JOURNAL, 2006, 48 (02) :217-227

[2] Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection [J].

Babu, Mohan ;

Gagarinova, Alla G. ;

Brandle, James E. ;

Wang, Aiming .

JOURNAL OF GENERAL VIROLOGY, 2008, 89 :1069-1080

[3] Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection [J].

Babu, Mohan ;

Griffiths, Jonathan S. ;

Huang, Tyng-Shyan ;

Wang, Aiming .

BMC GENOMICS, 2008, 9 (1)

[4] PVYNTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation [J].

Baebler, Spela ;

Krecic-Stres, Hana ;

Rotter, Ana ;

Kogovsek, Polona ;

Cankar, Katarina ;

Kok, Esther J. ;

Gruden, Kristina ;

Kovac, Maja ;

Zel, Jana ;

Pompe-Novak, Marusa ;

Ravnikar, Maja .

MOLECULAR PLANT PATHOLOGY, 2009, 10 (02) :263-275

[5] Decreased susceptibility to viral disease of beta-1,3-glucanase-deficient plants generated by antisense transformation [J].

Beffa, RS ;

Hofer, RM ;

Thomas, M ;

Meins, F .

PLANT CELL, 1996, 8 (06) :1001-1011

[6] ARACHIDONIC-ACID INDUCES LOCAL BUT NOT SYSTEMIC SYNTHESIS OF SALICYLIC-ACID AND CONFERS SYSTEMIC RESISTANCE IN POTATO PLANTS TO PHYTOPHTHORA-INFESTANS AND ALTERNARIA-SOLANI [J].

COQUOZ, JL ;

BUCHALA, AJ ;

MEUWLY, P ;

METRAUX, JP .

PHYTOPATHOLOGY, 1995, 85 (10) :1219-1224

[7] Transcriptional Changes and Oxidative Stress Associated with the Synergistic Interaction Between Potato virus X and Potato virus Y and Their Relationship with Symptom Expression [J].

Garcia-Marcos, Alberto ;

Pacheco, Remedios ;

Martianez, Justo ;

Gonzalez-Jara, Pablo ;

Ramon Diaz-Ruiz, Jose ;

Tenllado, Francisco .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2009, 22 (11) :1431-1444

[8] The oligopeptide elicitor Pep-13 induces salicylic acid-dependent and -independent defense reactions in potato [J].

Halim, VA ;

Hunger, A ;

Macioszek, V ;

Landgraf, P ;

Nürnberger, T ;

Scheel, D ;

Rosahl, S .

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2004, 64 (06) :311-318

[9] Salicylic acid is important for basal defense of solanum tuberosum against Phytophthora infestans [J].

Halim, Vincentius A. ;

Eschen-Lippold, Lennart ;

Altmann, Simone ;

Birschwilks, Mandy ;

Scheel, Dierk ;

Rosahl, Sabine .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2007, 20 (11) :1346-1352

[10] A defect in carbohydrate metabolism ameliorates symptom severity in virus-infected Arabidopsis thaliana [J].

Handford, Michael G. ;

Carr, John P. .

JOURNAL OF GENERAL VIROLOGY, 2007, 88 :337-341

← 1 2 3 4 5 →