Lactate dehydrogenase activity as an effect criterion in toxicity tests with Daphnia magna straus

被引：239

作者：

Diamantino, TC ^{[1
]}

Almeida, E

Soares, AMVM

Guilhermino, L

机构：

[1] Univ Coimbra, Inst Ambiente & Vida Largo Marques Pombal, Dept Zool, P-3000 Coimbra, Portugal

[2] Inst Nacl Engn & Tecnol Ind, LTR, P-1649038 Lisbon, Portugal

[3] Univ Porto, Inst Ciencias Biomed Abel Salazar, Dept Estudos Populacoes,Lab Ecotoxicol, Ctr Interdisciplinar Invest Marinha & Ambiental, P-4050 Oporto, Portugal

来源：

CHEMOSPHERE | 2001年 / 45卷 / 4-5期

关键词：

Daphnia magna; lactate dehydrogenase; biomarkers; zinc;

D O I：

10.1016/S0045-6535(01)00029-7

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Activity of lactate dehydrogenase (LDH) was used as an effect criterion in toxicity tests with Daphnia magna. In the first part of the work, the conditions for the use of LDH activity in toxicity tests with juveniles and adults of D. magna, were optimized. The influence of parameters such as the number of animals per sample, nutritional status, age and the presence of eggs in the brood chamber were investigated. In the second part of the study, both in vivo and in vitro tests based on the alteration of LDH activity of D. magna were developed and tested using zinc chloride as test substance. The results obtained indicate that LDH activity of D. magna may be used as an indicative parameter in aquatic toxicity tests. (C) 2001 Published by Elsevier Science Ltd.

引用

页码：553 / 560

页数：8

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