Human geranylgeranyl diphosphate synthase - cDNA cloning and expression

被引：56

作者：

Kuzuguchi, T ^{[1
]}

Morita, Y ^{[1
]}

Sagami, I ^{[1
]}

Sagami, H ^{[1
]}

Ogura, K ^{[1
]}

机构：

[1] Tohoku Univ, Inst Chem React Sci, Aoba Ku, Sendai, Miyagi 9808577, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 09期

关键词：

D O I：

10.1074/jbc.274.9.5888

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C-20-prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR.RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16%, sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA.

引用

页码：5888 / 5894

页数：7

共 36 条

[1]

CDNA CLONING OF COMPONENT-A OF RAB GERANYLGERANYL TRANSFERASE AND DEMONSTRATION OF ITS ROLE AS A RAB ESCORT PROTEIN [J].

ANDRES, DA ;

SEABRA, MC ;

BROWN, MS ;

ARMSTRONG, SA ;

SMELAND, TE ;

CREMERS, FPM ;

GOLDSTEIN, JL .

CELL, 1993, 73 (06) :1091-1099

[2]

ARMSTRONG SA, 1993, J BIOL CHEM, V268, P12221

[3]

BARTLETT DL, 1985, METHOD ENZYMOL, V110, P171

[4]

PROTEINS REGULATING RAS AND ITS RELATIVES [J].

BOGUSKI, MS ;

MCCORMICK, F .

NATURE, 1993, 366 (6456) :643-654

[5]

CHEN AJ, 1994, PROTEIN SCI, V3, P600

[6]

CLONING AND EXPRESSION OF A CDNA-ENCODING THE ALPHA SUBUNIT OF RAT P21RAS PROTEIN FARNESYLTRANSFERASE [J].

CHEN, WJ ;

ANDRES, DA ;

GOLDSTEIN, JL ;

BROWN, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11368-11372

[7]

MOLECULAR-CLONING AND SEQUENCE OF A CHOLESTEROL-REPRESSIBLE ENZYME RELATED TO PRENYLTRANSFERASE IN THE ISOPRENE BIOSYNTHETIC-PATHWAY [J].

CLARKE, CF ;

TANAKA, RD ;

SVENSON, K ;

WAMSLEY, M ;

FOGELMAN, AM ;

EDWARDS, PA .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (09) :3138-3146

[8]

PROTEIN ISOPRENYLATION AND METHYLATION AT CARBOXYL-TERMINAL CYSTEINE RESIDUES [J].

CLARKE, S .

ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :355-386

[9]

DAVISSON VJ, 1985, METHOD ENZYMOL, V110, P130

[10]

PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN FARNESYL DIPHOSPHATE SYNTHASE EXPRESSED IN ESCHERICHIA-COLI [J].

DING, VDH ;

SHEARES, BT ;

BERGSTROM, JD ;

PONPIPOM, MM ;

PEREZ, LB ;

POULTER, CD .

BIOCHEMICAL JOURNAL, 1991, 275 :61-65

← 1 2 3 4 →