Evidence for Msh2 haploinsufficiency in mice revealed by MNU-induced sister-chromatid exchange analysis

被引：15

作者：

Bouffler, SD ^{[1
]}

Hofland, N

Cox, R

Fodde, R

机构：

[1] Natl Radiol Protect Board, Radiat Effects Dept, Didcot OX11 0RQ, Oxon, England

[2] Leiden Univ, Med Ctr, Dept Human & Clin Genet, NL-2300 RA Leiden, Netherlands

来源：

BRITISH JOURNAL OF CANCER | 2000年 / 83卷 / 10期

关键词：

Msh2; mismatch repair; alkylating agent; ionizing radiation; sister-chromatid exchange;

D O I：

10.1054/bjoc.2000.1422

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The role of Msh2 in chromosome stability has been investigated in a targeted mouse model for HNPCC, Msh2 Delta 7N. Chromosome aberration frequencies were similar in bone marrow of Msh2(+/+), Msh2(+/-) and Msh2(-/-) mice and no differential effects of in vivo X-irradiation were noted. By contrast, the induction of sister-chromatid exchanges (SCEs) by methyl nitrosourea (MNU) was reduced in Msh2(-/-) and Msh2(+/-) cells to similar to 20% and similar to 45% wild-type levels respectively indicating a phenotypic effect of haploinsufficiency of the mouse Msh2 gene. (C) 2000 Cancer Research Campaign.

引用

页码：1291 / 1294

页数：4

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