A simple method for the serodiagnosis of human hydatid disease based on a protein A colloidal dye conjugate

被引：17

作者：

Eliades, P

Karagouni, E

Stergiatou, I

Miras, K

机构：

[1] BIO CHECK INT, Med Ctr, Athens, Greece

[2] Hellen Pasteur Inst, Athens 11521, Greece

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1998年 / 218卷 / 1-2期

关键词：

hydatid disease; Echinococcus; protein A; colloidal dye; immunodot assay;

D O I：

10.1016/S0022-1759(98)00122-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a dot immunobinding assay (CM) for the serodiagnosis of the human hydatidosis. The assay employs Samaron blue colloidal dye particles conjugated to hydatid antigen (HA) or protein A, both of which serve as visualizing agents. The test is simple and can be completed in 1 h. It requires few reagents, a nitrocellulose membrane (NC) strip onto which the HA has been bound, and the colored conjugates. It is sensitive and specific for the detection of anti-HA antibodies and generally agrees closely with the data from the enzyme-linked immunosorbent assay (ELISA). (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 132

页数：10

共 21 条

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