Effect of induced hypertension with angiotensin II infusion on biodistribution of In-111-labeled monoclonal antibody

We investigated whether induced hypertension could enhance tumor uptake of monoclonal antibody. In-111-DTPA-A7 (IgG, against 40kD tumor associated glycoprotein) was injected into colon carcinoma xenografted mice which were subcutaneously implanted with micro-osmotic pump containing angiotensin II (AT-II). Biodistribution was observed in groups of mice infused with AT-II at rate of 0.5 mu g/kg/min (L) or 1 mu g/kg/min (H) and compared with a group of mice infused with saline (S). Tumor uptake of In-111-A7 in L and H was 1.32 and 1.57 times greater than S at 48 h after intravenous injection of A7. Normal organ uptakes also tended to be increased by AT II infusion. Further study is needed to get optimum effect of hypertensive treatment on biodistribution of radiolabeled MoAb.