Cloning and expression of a Ralstonia eutropha HF39 gene mediating indigo formation in Escherichia coli

被引：19

作者：

Drewlo, S

Brämer, CO

Madkour, M

Mayer, F

Steinbüchel, A

机构：

[1] Univ Munster, Inst Mikrobiol, D-48149 Munster, Germany

[2] Univ Gottingen, Inst Mikrobiol & Genet, D-37073 Gottingen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 04期

关键词：

D O I：

10.1128/AEM.67.4.1964-1969.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

On complex medium Escherichia coli strains carrying hybrid plasmid pBEC/EE:11.0, pSKBEC/BE:9.0, pSKBEC/PP:3.3, or pSKBEC/PP:2.4 harboring genomic DNA of Ralstonia eutropha HF39 produced a blue pigment characterized as indigo by several chemical and spectroscopic methods. A 1,251-bp open reading frame (bec) was cloned and sequenced. The deduced amino acid sequence of bec showed only weak similarities to short-chain acyl-coenzyme A dehydrogenases, and the gene product catalyzed formation of indoxyl, a reactive preliminary stage for production of indigo.

引用

页码：1964 / 1969

页数：6

共 24 条

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