Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene

被引：105

作者：

Pedraza-Díaz, S

Amar, C

Nichols, GL

McLauchlin, J

机构：

[1] PHLS, Cent Publ Hlth Lab, Food Safety Microbiol Lab, London NW9 5HT, England

[2] PHLS, Ctr Communicable Dis Surveillance, London, England

来源：

EMERGING INFECTIOUS DISEASES | 2001年 / 7卷 / 01期

关键词：

D O I：

10.3201/eid0701.010109

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.

引用

页码：49 / 56

页数：8

共 37 条

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