Mammalian mitochondrial DNA replicates bidirectionally from an initiation zone

被引：150

作者：

Bowmaker, M

Yang, MY

Yasukawa, T

Reyes, A

Jacobs, HT

Huberman, JA

Holt, IJ

机构：

[1] Dunn Human Nutr Unit, Cambridge CB2 2XY, England

[2] CNR, Ist Tecnol Biomed, Sez Bioinformat & Genom Bari, I-70126 Bari, Italy

[3] Univ Tampere, Inst Med Technol, FIN-33014 Tampere, Finland

[4] Univ Tampere, Tampere Univ Hosp, FIN-33014 Tampere, Finland

[5] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland

[6] Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 51期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1074/jbc.M308028200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Previous data from our laboratory suggested that replication of mammalian mitochondrial DNA initiates exclusively at or near to the formerly designated origin of heavy strand replication, O-H, and proceeds unidirectionally from that locus. New results obtained using two-dimensional agarose gel electrophoresis of replication intermediates demonstrate that replication of mitochondrial DNA initiates from multiple origins across a broad zone. After fork arrest near O-H, replication is restricted to one direction only. The initiation zone of bidirectional replication includes the genes for cytochrome b and NADH dehydrogenase subunits 5 and 6.

引用

页码：50961 / 50969

页数：9

共 46 条

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Replication initiation patterns in the β-globin loci of totipotent and differentiated murine cells:: Evidence for multiple initiation regions [J].