Economical and efficient overproduction of the Escherichia coli sigma 32 transcription factor

被引：2

作者：

Kotlarz, A

Szalewska-Palasz, A

Wegrzyn, G

Lipinska, B

机构：

[1] Univ Gdansk, Dept Biochem, PL-80822 Gdansk, Poland

[2] Univ Gdansk, Dept Mol Biol, PL-80822 Gdansk, Poland

[3] Polish Acad Sci, Marine Biol Ctr, Mol Biol Lab, PL-80822 Gdansk, Poland

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 12期

关键词：

E-coli; sigma; 32; heat shock;

D O I：

10.1023/A:1008853209547

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sigma 32 of Escherichia coli, encoded by the rpoH gene, is a transcription factor enabling RNA polymerase to recognize promoters of heat shock genes. The rpoH gene was cloned in the bacteriophage T7 RNA polymerase/ promoter system and expressed in the E. coli ftsH mutant. In this, heat-inducible and economical system, we obtained overproduction of sigma32 and the purified protein was active in the in vitro transcription assay.

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收藏

页码：869 / 873

页数：5

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