Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event

被引：69

作者：

Kumar, KP ^{[1
]}

Akoulitchev, S ^{[1
]}

Reinberg, D ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Biochem, Howard Hughes Med Inst,Div Nucl Acid Enzymol, Piscataway, NJ 08854 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 17期

关键词：

D O I：

10.1073/pnas.95.17.9767

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The accumulation of promoter-proximal stalled complexes was reduced in the presence of TFIIH and efficient escape from this site also required an activator. Promoter-proximal stalled complexes could not be induced to resume elongation. Our results indicate that effective recruitment of TFIIH into transcription complexes is achieved during formation of the preinitiation complex at the promoter. The studies establish that promoter clearance is a regulated event that requires TFIIH.

引用

页码：9767 / 9772

页数：6

共 38 条

[1] Distinct activated and non-activated RNA polymerase II complexes in yeast [J].