Application of the ovarian oil injection and ovariolar separation techniques for age grading hematophagous diptera

被引：4

作者：

Hoc, TQ ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,LEHRSTUHL SPEZIELLE ZOOL & PARASITOL,D-44780 BOCHUM,GERMANY

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 1996年 / 33卷 / 03期

关键词：

hematophagous Diptera; ovarian oil injection technique; ovariolar separation technique; age grading; granular basal body; dilatations;

D O I：

10.1093/jmedent/33.3.290

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The ovarian oil injection and ovariolar separation techniques were applied to determine the physiological age of species of tie 3 dipteran families Culicidae, Simuliidae, and Tabanidae. The presence of a granular basal body (a granular area in tile calyx wall within the ovariolar sheath) indicates at least 1 egg laying; that is, it cim be used to separate parous from nulliparous females, which possess no granular basal body. In parous females, the number of ovipositions can be determined accurately by counting the number of dilatations in the diagnostic ovarioles. An egg sac, when present, indicates only the current egg laying. A diagram for age grading using the technique is presented and discussed.

引用

页码：290 / 296

页数：7

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