Development and evaluation of a dipstick immunoassay format for the determination of atrazine residues on-site

被引：39

作者：

Wittmann, C

Bilitewski, U

Giersch, T

Kettling, U

Schmid, RD

机构：

[1] UNIV STUTTGART,INST TECH BIOCHEM,D-70569 STUTTGART,GERMANY

[2] GBF,PROJECT GRP BIOSENSORS,D-38124 BRAUNSCHWEIG,GERMANY

[3] TECH UNIV MUNICH,DEPT BOT,D-85350 FREISING,GERMANY

来源：

ANALYST | 1996年 / 121卷 / 06期

关键词：

atrazine; dipstick immunoassay; enzyme immunoassay; pesticide residue; water sample;

D O I：

10.1039/an9962100863

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On the basis of a semi-quantitative dipstick immunoassay (IA) for atrazine with visual detection (Giersch, T., J. Agric. Food Chem., 1993, 41, 1006), a quantitative format suitable as a field assay for the analysis of pesticide residues in water and liquid food samples on-site is described. For antibody immobilization, different membranes and immobilization techniques were investigated. The measuring range for atrazine was 0.3-10 mu g l(-1) using reflectance detection. The total assay time was about 25 min with dipsticks previously coated with antibody. Atrazine-spiked water and liquid food samples were selected for assay evaluation. The samples could be measured directly without the need for any prior enrichment or clean-up steps. A satisfactory agreement was found between the results of the dipstick IA and HPLC or GC measurements of both the original and spiked samples.

引用

页码：863 / 869

页数：7

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