Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism

被引：268

作者：

Casais, R ^{[1
]}

Dove, B ^{[1
]}

Cavanagh, D ^{[1
]}

Britton, P ^{[1
]}

机构：

[1] Inst Anim Hlth, Div Mol Biol, Compton Lab, Newbury RG20 7NN, Berks, England

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 16期

关键词：

D O I：

10.1128/JVI.77.16.9084-9089.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75:12359-12369, 2001), in which the ectodomain region of the spike gene from IBV M41-CK replaced the corresponding region of the IBV Beaudette genome. BeauR-M41(S) acquired the same cell tropism phenotype as IBV M41-CK in four different cell types, demonstrating that the IBV spike glycoprotein is a determinant of cell tropism.

引用

页码：9084 / 9089

页数：6

共 24 条

[1]

BRITTON P, 1991, NATURE, V353, P394, DOI 10.1038/353394a0

[2]

Reverse genetics system for the avian coronavirus infectious bronchitis virus [J].

Casais, R ;

Thiel, V ;

Siddell, SG ;

Cavanagh, D ;

Britton, P .

JOURNAL OF VIROLOGY, 2001, 75 (24) :12359-12369

[3]

Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys [J].

Cavanagh, D ;

Mawditt, K ;

Welchman, DD ;

Britton, P ;

Gough, RE .

AVIAN PATHOLOGY, 2002, 31 (01) :81-93

[4]

Detection of a coronavirus from turkey poults in Europe genetically related to infectious bronchitis virus of chickens [J].

Cavanagh, D ;

Mawditt, K ;

Sharma, M ;

Drury, SE ;

Ainsworth, HL ;

Britton, P ;

Gough, RE .

AVIAN PATHOLOGY, 2001, 30 (04) :355-368

[5]

A nomenclature for avian coronavirus isolates and the question of species status [J].

Cavanagh, D .

AVIAN PATHOLOGY, 2001, 30 (02) :109-115

[6]

Cavanagh D., 2003, DIS POULTRY, V11th, P101

[7]

Protection of chickens against renal damage caused by a nephropathogenic infectious bronchitis virus [J].

Cook, JKA ;

Chesher, J ;

Baxendale, W ;

Greenwood, N ;

Huggins, MB ;

Orbell, SJ .

AVIAN PATHOLOGY, 2001, 30 (04) :423-426

[8]

TAXONOMIC STUDIES ON STRAINS OF AVIAN INFECTIOUS-BRONCHITIS VIRUS USING NEUTRALIZATION TESTS IN TRACHEAL ORGAN-CULTURES [J].

DARBYSHIRE, JH ;

ROWELL, JG ;

COOK, JKA ;

PETERS, RW .

ARCHIVES OF VIROLOGY, 1979, 61 (03) :227-238

[9]

Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus [J].

Das Sarma, J ;

Fu, L ;

Tsai, JC ;

Weiss, SR ;

Lavi, E .

JOURNAL OF VIROLOGY, 2000, 74 (19) :9206-9213

[10]

The genome organization of the nidovirales: Similarities and differences between arteri-, toro-, and coronaviruses [J].

de Vries, AAF ;

Horzinek, MC ;

Rottier, PJM ;

de Groot, RJ .

SEMINARS IN VIROLOGY, 1997, 8 (01) :33-47

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