Fingerprinting of glycans as their 2-aminoacridone derivatives by capillary electrophoresis and laser-induced fluorescence

被引：21

作者：

Harland, GB

Okafo, G

Matejtschuk, P

Sellick, IC

Chapman, GE

Camilleri, P

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,WELWYN GARDEN CIT AL6 9AR,HERTS,ENGLAND

[2] BIOPROD LAB,ELSTREE,HERTS,ENGLAND

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 02期

关键词：

glycans; fetuin; monoclonal antibodies; capillary electrophoresis; 2-aminoacridone;

D O I：

10.1002/elps.1150170220

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis and laser-induced fluorescence detection have been used to fingerprint the 2-aminoacridone derivatives of complex glycans released from bovine fetuin and human IgG monoclonal antibodies. The utility of this method in distinguishing between N- and O-linked oligosaccharides and in determining the presence of sialic acid residues in glycan mixtures at an early stage of analysis has been demonstrated.

引用

收藏

页码：406 / 411

页数：6

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