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Robust hepatitis C virus infection in vitro

被引：1410
作者：
Zhong, J
Gastaminza, P
Cheng, GF
Kapadia, S
Kato, T
Burton, DR
Wieland, SF
Uprichard, SL
Wakita, T
Chisari, FV
机构：
[1] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Dept Immunol, La Jolla, CA 92037 USA
[3] Tokyo Metropolitan Inst Neurosci, Dept Microbiol, Tokyo 1838526, Japan
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 26期
关键词：
CD81; Huh-7; viral entry; viral spread; interferon;
D O I：
10.1073/pnas.0503596102
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.
引用
收藏
页码：9294 / 9299
页数：6
相关论文
共 51 条
[1]   Recovery, persistence, and sequelae in hepatitis C virus infection: A perspective on long-term outcome [J].
Alter, HJ ;
Seeff, LB .
SEMINARS IN LIVER DISEASE, 2000, 20 (01) :17-35
[2]   Impaired dendritic cell maturation in patients with chronic, but not resolved, hepatitis C virus infection [J].
Auffermann-Gratzinger, S ;
Keeffe, EB ;
Levy, S .
BLOOD, 2001, 97 (10) :3171-3176
[3]   Replication of the hepatitis C virus in cell culture [J].
Bartenschlager, R ;
Kaul, A ;
Sparacio, S .
ANTIVIRAL RESEARCH, 2003, 60 (02) :91-102
[4]   Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes [J].
Bartosch, B ;
Dubuisson, J ;
Cosset, FL .
JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 197 (05) :633-642
[5]   Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication [J].
Blight, KJ ;
McKeating, JA ;
Rice, CM .
JOURNAL OF VIROLOGY, 2002, 76 (24) :13001-13014
[6]   Efficient initiation of HCV RNA replication in cell culture [J].
Blight, KJ ;
Kolykhalov, AA ;
Rice, CM .
SCIENCE, 2000, 290 (5498) :1972-1974
[7]   Mutations that permit efficient replication of hepatitis C virus RNA in Huh-7 cells prevent productive replication in chimpanzees [J].
Bukh, J ;
Pietschmann, T ;
Lohmann, V ;
Krieger, N ;
Faulk, K ;
Engle, RE ;
Govindarajan, S ;
Shapiro, M ;
Claire, MS ;
Bartenschlager, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) :14416-14421
[8]   A critical role for the chimpanzee model in the study of hepatitis C [J].
Bukh, J .
HEPATOLOGY, 2004, 39 (06) :1469-1475
[9]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10]   CD81 is an entry coreceptor for hepatitis C virus [J].
Cormier, EG ;
Tsamis, F ;
Kajumo, F ;
Durso, RJ ;
Gardner, JP ;
Dragic, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (19) :7270-7274
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