Protein kinase G II-mediated proliferative effects in human cultured prostatic stromal cells

被引：52

作者：

Cook, ALM ^{[1
]}

Haynes, JM ^{[1
]}

机构：

[1] Monash Univ, Victorian Coll Pharm, Dept Pharmaceut Biol & Pharmacol, Melbourne, Vic 3052, Australia

来源：

CELLULAR SIGNALLING | 2004年 / 16卷 / 02期

基金：

英国医学研究理事会;

关键词：

prostatic stromal cells; protein kinase G; cyclic GMP; proliferation; apoptosis;

D O I：

10.1016/S0898-6568(03)00134-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study investigates the effect of protein kinase G (PKG) activation upon proliferation of human cultured prostatic stromal cells. The PKG II activator (8-pCPT-cGMP; IC50 of 113 +/- 42 nM) and the phosphodiesterase inhibitor, zaprinast (up to 50 muM), but not the PKG I isoform activators (APT-cGMP and PET-cGMP), reduced foetal calf serum-stimulated proliferation. The effect of 8-pCPT-cGMP (30 muM) was blocked by Rp-8-Br-cGMPS (5 muM) and Rp-8-pCPT-cGMP (5 muM), but not Rp-cAMPS (5 muM). 8-pCPT-cGMP (30 muM) and zaprinast (50 muM), but not PET-cGMP (30 muM), caused a significant increase in atypical nuclei and an increase in annexin-V staining. These data indicate that activation of PKG II induces apoptosis of human cultured prostatic stromal cells. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：253 / 261

页数：9

共 62 条

[1] Lysophosphatidic acid stimulates proliferation of cultured smooth muscle cells from human BPH tissue: Sildenafil and papaverin generate inhibition [J].