Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

被引：25

作者：

Satinover, DL

Vance, GH

Van Dyke, DL

Schwartz, S ^{[1
]}

机构：

[1] Case Western Reserve Univ, Sch Med, Dept Genet, Cleveland, OH 44106 USA

[2] Case Western Reserve Univ, Sch Med, Ctr Human Genet, Cleveland, OH 44106 USA

[3] Univ Hosp Cleveland, Cleveland, OH 44106 USA

[4] Indiana Univ, Sch Med, Dept Med & Mol Genet, Indianapolis, IN 46204 USA

[5] Henry Ford Hosp, Detroit, MI 48202 USA

来源：

CHROMOSOMA | 2001年 / 110卷 / 04期

关键词：

D O I：

10.1007/s004120100143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over 40 cases of neocentric marker chromosomes, without detectable a-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentromeric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared. with the average levels in the genome.

引用

页码：275 / 283

页数：9

共 17 条

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