Transcriptional regulation of Salmonella virulence: A phoQ periplasmic domain mutation results in increased net phosphotransfer to phoP

被引：76

作者：

Gunn, JS

Hohmann, EL

Miller, SI

机构：

[1] UNIV WASHINGTON, DEPT MED, SEATTLE, WA 98195 USA

[2] UNIV WASHINGTON, DEPT MICROBIOL, SEATTLE, WA 98195 USA

[3] MASSACHUSETTS GEN HOSP, INFECT DIS UNIT, BOSTON, MA 02114 USA

[4] HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 21期

关键词：

D O I：

10.1128/jb.178.21.6369-6373.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A mutation in the phoP/phoQ locus (pho-24) that results in unregulated expression of PhoP-activated genes (phenotype PhoP constitutive [PhoP(c)]) was mapped to phoQ. Change of a Thr to Ile at position 48 of PhoQ was responsible for the PhoP(c) phenotype (attenuation of mouse virulence, defects in epithelial cell invasion, and macrophage spacious phagosome formation). PhoP phosphorylation by membrane extracts required PhoQ, and PhoQ Ile-48-containing membranes demonstrated increased net phosphorylation of PhoP.

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页码：6369 / 6373

页数：5

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