Adenovirus E4-34kDa requires active proteasomes to promote late gene expression

被引:27
作者
Corbin-Lickfett, KA [1 ]
Bridge, E [1 ]
机构
[1] Miami Univ, Dept Microbiol, Oxford, OH 45056 USA
关键词
Adenovirus; E4; ORF6; E4-34kDa; E1b-55kDa; ORF3; E4-11kDa; 26S proteasome; MG132; lactacystin;
D O I
10.1016/S0042-6822(03)00527-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A complex of the Adenovirus (Ad) early region 1b 55-kDa protein (E1b-55kDa) and the early region 4 ORF6 34-kDa protein (E4-34kDa) promotes viral late RNA accumulation in the cytoplasm while inhibiting the transport of most newly synthesized cellular mRNA. The E4 ORF3 11-kDa protein (E4-11kDa) functionally compensates for at least some of the activities of this complex. We find that the same large central region of E4-34kDa that is required for proteasome-mediated degradation of p53 (J. Virol. 75, (2001) 699-709) is also required to promote viral late gene expression in a complementation assay. E4-34kDa does not promote late gene expression in complementation assays performed in the presence of proteasome inhibitors. A proteasome inhibitor also dramatically reduced late gene expression by a virus that lacks the E4-11kDa gene and therefore relies on E4-34kDa for late gene expression. Our results suggest that E4-34kDa activity in promoting late gene expression depends on the proteasome. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:234 / 244
页数:11
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