共 40 条
Adenovirus E4-34kDa requires active proteasomes to promote late gene expression
被引:27
作者:
Corbin-Lickfett, KA
[1
]
Bridge, E
[1
]
机构:
[1] Miami Univ, Dept Microbiol, Oxford, OH 45056 USA
来源:
关键词:
Adenovirus;
E4;
ORF6;
E4-34kDa;
E1b-55kDa;
ORF3;
E4-11kDa;
26S proteasome;
MG132;
lactacystin;
D O I:
10.1016/S0042-6822(03)00527-0
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
A complex of the Adenovirus (Ad) early region 1b 55-kDa protein (E1b-55kDa) and the early region 4 ORF6 34-kDa protein (E4-34kDa) promotes viral late RNA accumulation in the cytoplasm while inhibiting the transport of most newly synthesized cellular mRNA. The E4 ORF3 11-kDa protein (E4-11kDa) functionally compensates for at least some of the activities of this complex. We find that the same large central region of E4-34kDa that is required for proteasome-mediated degradation of p53 (J. Virol. 75, (2001) 699-709) is also required to promote viral late gene expression in a complementation assay. E4-34kDa does not promote late gene expression in complementation assays performed in the presence of proteasome inhibitors. A proteasome inhibitor also dramatically reduced late gene expression by a virus that lacks the E4-11kDa gene and therefore relies on E4-34kDa for late gene expression. Our results suggest that E4-34kDa activity in promoting late gene expression depends on the proteasome. (C) 2003 Elsevier Inc. All rights reserved.
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页码:234 / 244
页数:11
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