Artificial imitation of glutathione peroxidase with 6-selenium-bridged β-cyclodextrin

被引：68

作者：

Liu, JQ

Gao, SJ

Luo, GM ^{[1
]}

Yan, GL

Shen, JC

机构：

[1] Jilin Univ, Natl Lab Enzyme Engn, Changchun 130023, Peoples R China

[2] Jilin Univ, Key Lab Supermol Spectra & Struct, Changchun 130023, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 247卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.8545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

On the basis of cyclodextrin, 6-selenium bridged beta-cyclodextrin (6-beta-CD-Se-Se-beta-CD, known as 6-SeCD) was synthesized by the selective tosylation of beta-cyclodextrin and nucleophilic displacement by sodium hydroselenide to imitate glutathione peroxidase (GPX). The GPX activity of diselenide 6-SeCD is 4.3 times that of PZ51. The structure of the mimic 6-SeCD was characterized by means of laser mass spectroscopy, elemental analysis, IR and H-1 NMR. The selenium content and its valence in 6-SeCD were determined by means of X-ray photoelectron spectra. Kinetics of the mimic showed that its enzymatic behavior was similar to that of native GPX. (C) 1998 Academic Press.

引用

页码：397 / 400

页数：4

共 15 条

[1]

BENER ML, 1978, CYCLODEXTRIN CHEM

[2]

CHIU DY, 1976, BIOCHIM BIOPHYS ACTA, V455, P558

[3] EXPEDIENT SYNTHESIS OF EBSELEN AND RELATED-COMPOUNDS [J].