Growth Inhibition Effect of β-Catenin Small Interfering RNA-Mediated Gene Silencing on Human Colon Carcinoma HT-29 Cells

被引：12

作者：

Xu, Wei-Li ^{[2
]}

Wang, Qi ^{[1
]}

Du, Ming ^{[2
]}

Zhao, Yong-Huan ^{[3
]}

Sun, Xiang-Rong ^{[1
]}

Sun, Wen-Guang ^{[1
]}

Chen, Bing-Qing ^{[1
]}

机构：

[1] Harbin Med Coll, Dept Nutr & Food Hyg, Coll Publ Hlth, Harbin 150081, Peoples R China

[2] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150006, Peoples R China

[3] Heilongjiang August First Land Reclamat Univ, Dept Food Sci, Daqing, Peoples R China

来源：

CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS | 2010年 / 25卷 / 05期

基金：

中国国家自然科学基金;

关键词：

colon cancer; gene therapy; HT-29; RNA interference; Wnt/beta-catenin; DOUBLE-STRANDED-RNA; C-MYC; CANCER CELLS; CYCLIN D1; COLORECTAL-CANCER; INDUCED APOPTOSIS; IN-VIVO; EXPRESSION; APC; TRANSCRIPTION;

D O I：

10.1089/cbr.2010.0768

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The beta-catenin gene is a critical component of Wnt signaling pathway. Aberrant activation of Wnt/beta-catenin signaling and subsequent upregulation of beta-catenin is related to enhancing cell proliferation and developing colon polyps and colon cancer. In the present study, the effect of beta-catenin knockdown on the growth and survival of the human colon cancer cell line HT-29 was investigated in vitro. The effect of knockdown of beta-catenin on cell proliferation was investigated by MTT assay and colony formation. The cell cycle distribution was investigated by flow cytometry. Apoptosis was measured by nuclear staining and flow cytometry. The change of beta-catenin and related proteins were determined by western blotting and immunofluorescence. The results showed that small interfering RNA directed against beta-catenin markedly inhibited the expression and nuclear translocation of beta-catenin and decreased the expression of known target genes such as cyclin D1 and c-myc; HT-29 cell proliferation was inhibited as indicated by growth reduction, cell cycle arrest in G0/G1 phase, and induction of apoptosis; and the inhibition of cell growth may be associated with switching off cyclin D1 and c-myc expression by small interfering RNA targeted against beta-catenin in colon cancer HT-29 cells.

引用

页码：529 / 537

页数：9

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