Application of tRNA intergenic spacer PCR for identification of Enterococcus species

被引：170

作者：

Baele, M

Baele, P

Vaneechoutte, M

Storms, V

Butaye, P

Devriese, LA

Verschraegen, G

Gillis, M

Haesebrouck, F

机构：

[1] State Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Poultry Dis, B-9820 Merelbeke, Belgium

[2] State Univ Ghent, Fac Med, Dept Clin Chem Microbiol & Immunol, B-9000 Ghent, Belgium

[3] State Univ Ghent, Fac Wetenschappen, Microbiol Lab, B-9000 Ghent, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 11期

关键词：

D O I：

10.1128/JCM.38.11.4201-4207.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.

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页码：4201 / 4207

页数：7

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