Molecular cloning and sequencing of cDNAs encoding insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Simon)

被引：14

作者：

Leisy, DJ ^{[1
]}

Mattson, JD ^{[1
]}

Quistad, GB ^{[1
]}

Kramer, SJ ^{[1
]}

VanBeek, N ^{[1
]}

Tsai, LW ^{[1
]}

Enderlin, FE ^{[1
]}

Woodworth, AR ^{[1
]}

Digan, ME ^{[1
]}

机构：

[1] SANDOZ AGRO INC,PALO ALTO,CA 94304

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1996年 / 26卷 / 05期

关键词：

Plectreurys tristis; insecticidal toxins; PCR;

D O I：

10.1016/0965-1748(95)00070-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plectreurys tristis cephalothorax mRNA was isolated and amplified by PCR using degenerate primers corresponding to reverse translated mature Plt-VI toxin, An oligonucleotide corresponding to a portion of the amplified product was then used to screen a P. tristis cDNA library, The cDNAs from 10 positive clones were sequenced, Eight of these cDNAs corresponded to Plt-VI toxin, one to Plt-XI toxin, and one was very similar to Plt-VIII toxin, with the exception of a single amino acid substitution. Analysis of these cDNAs indicated that these toxins are initially synthesized as prepro-forms which undergo signal cleavage followed by additional processing at both their N- and C-termini to produce the mature products. Copyright (C) 1996 Elsevier Science Ltd.

引用

页码：411 / 417

页数：7

共 18 条

[1] LOCATION, SEQUENCE, TRANSCRIPTIONAL MAPPING, AND TEMPORAL EXPRESSION OF THE GP64 ENVELOPE GLYCOPROTEIN GENE OF THE ORGYIA-PSEUDOTSUGATA MULTICAPSID NUCLEAR POLYHEDROSIS-VIRUS [J].