ARFGAP1 promotes AP-2-dependent endocytosis

被引：30

作者：

Bai, Ming ^{[1
,2
]}

Gad, Helge ^{[3
,4
]}

Turacchio, Gabriele ^{[3
,5
]}

Cocucci, Emanuele ^{[6
,7
]}

Yang, Jia-Shu ^{[1
,2
]}

Li, Jian ^{[1
,2
]}

Beznoussenko, Galina V. ^{[3
]}

Nie, Zhongzhen ^{[8
]}

Luo, Ruibai ^{[9
]}

Fu, Lianwu ^{[10
]}

Collawn, James F. ^{[10
]}

Kirchhausen, Tomas ^{[6
,7
]}

Luini, Alberto ^{[3
,11
]}

Hsu, Victor W. ^{[1
,2
]}

机构：

[1] Harvard Univ, Brigham & Womens Hosp, Div Rheumatol Immunol & Allergy, Sch Med, Boston, MA 02115 USA

[2] Harvard Univ, Dept Med, Sch Med, Boston, MA 02115 USA

[3] Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Chieti, Italy

[4] Stockholm Univ, Dept Genet Microbiol & Toxicol, S-10691 Stockholm, Sweden

[5] CNR, Inst Prot Biochem, I-80131 Naples, Italy

[6] Harvard Univ, Dept Cell Biol, Sch Med, Boston, MA 02115 USA

[7] Immune Dis Inst, Boston, MA 02115 USA

[8] Univ Florida Coll Med, Dept Urol, Gainesville, FL 32610 USA

[9] NCI, Cellular & Mol Biol Lab, Bethesda, MD 20892 USA

[10] Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA

[11] Telethon Inst Genet & Med, I-80131 Naples, Italy

来源：

NATURE CELL BIOLOGY | 2011年 / 13卷 / 05期

基金：

美国国家卫生研究院;

关键词：

TRANSMEMBRANE CONDUCTANCE REGULATOR; CLATHRIN-MEDIATED ENDOCYTOSIS; COPI VESICLE FORMATION; TRANSFERRIN RECEPTOR; SORTING SIGNALS; GOLGI TRANSPORT; ENDOPLASMIC-RETICULUM; ADAPTER COMPLEX; ALPHA-APPENDAGE; COATED VESICLES;

D O I：

10.1038/ncb2221

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

COPI (coat protein I) and the clathrin-AP-2 (adaptor protein 2) complex are well-characterized coat proteins, but a component that is common to these two coats has not been identified. The GTPase-activating protein (GAP) for ADP-ribosylation factor 1 (ARF1), ARFGAP1, is a known component of the COPI complex. Here, we show that distinct regions of ARFGAP1 interact with AP-2 and coatomer (components of the COPI complex). Selectively disrupting the interaction of ARFGAP1 with either of these two coat proteins leads to selective inhibition in the corresponding transport pathway. The role of ARFGAP1 in AP-2-regulated endocytosis has mechanistic parallels with its roles in COPI transport, as both its GAP activity and coat function contribute to promoting AP-2 transport.

引用

页码：559 / U144

页数：20

共 58 条

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INTERMOLECULAR DISULFIDE BONDS ARE NOT REQUIRED FOR THE EXPRESSION OF THE DIMERIC STATE AND FUNCTIONAL-ACTIVITY OF THE TRANSFERRIN RECEPTOR [J].