Production and characterization of mouse monoclonal antibodies reactive to Chikungunya envelope E2 glycoprotein

被引：50

作者：

Brehin, Anne-Claire ^{[1
]}

Rubrecht, Laetitia ^{[2
]}

Navarro-Sanchez, Martha Erika ^{[1
]}

Marechal, Valerie ^{[1
]}

Frenkiel, Marie-Pascale ^{[1
]}

Lapalud, Priscilla ^{[2
]}

Laurie, Daniel ^{[2
]}

Sall, Amadou Alpha ^{[3
]}

Despres, Philippe ^{[1
]}

机构：

[1] Inst Pasteur, Dept Virol, Unite Interact Mol Flavivirus Hotes, F-75724 Paris, France

[2] CNRS, FRE 3009, Montpellier, France

[3] Inst Pasteur, Unite Arbovirus & Virus Fievres Hemorrag, Dakar, Senegal

来源：

VIROLOGY | 2008年 / 371卷 / 01期

关键词：

Chikungunya virus; alphaviruses; envelope E2 glycoprotein; recombinant E2 antigen; monoclonal antibodies; indirect ELISA; antigen-capture ELISA; immunufluorescence assay; immunoblot analysis; viral diagnosis;

D O I：

10.1016/j.virol.2007.09.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chikungunya fever is an arbovirosis of major impact in public health in Asia and Africa. Chikungunya (CHIK) virus is member of the genus Alphavirus and belongs to the Semliki Forest (SF) antigenic complex. We describe for the first time a panel of monoclonal antibodies (MAbs) reactive to CHIK envelope E2 glycoprotein. For the screening of E2-specific MAbs, we expressed a recombinant soluble CHIK E2 protein in Drosophila S2 cells. Analyzed by immunological methods, MAbs 30, 3E4, and 8A4 were selected on the basis of their reactivity. Their epitopes are located to the outer surface of CHIK virion. These MAbs have no cross reactivity with related members of SF antigenic complex with the notable exception of Igbo-Ora virus. Anti-CHIK E2 MAbs 30, 3E4, and 8A4 should be helpful for studying the biology of CHIK virus and pathogenesis of disease. The combination of 8A4 and 3E4 is suitable for developing a specific antigen-capture ELISA. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：185 / 195

页数：11

共 26 条

[1] Genetic divergence of Chikungunya viruses in India (1963-2006) with special reference to the 2005-2006 explosive epidemic [J].

Arankalle, Vidya A. ;

Shrivastava, Shubham ;

Cherian, Sarah ;

Gunjikar, Rashmi S. ;

Walimbe, Atul M. ;

Jadhav, Santosh M. ;

Sudeep, A. B. ;

Mishra, Akhilesh C. .

JOURNAL OF GENERAL VIROLOGY, 2007, 88 :1967-1976

[2] ANTIGENIC RELATIONSHIP BETWEEN CHIKUNGUNYA VIRUS-STRAINS AND ONYONG NYONG VIRUS USING MONOCLONAL-ANTIBODIES [J].

BLACKBURN, NK ;

BESSELAAR, TG ;

GIBSON, G .

RESEARCH IN VIROLOGY, 1995, 146 (01) :69-73

[3]

Borgehrini G., 2007, J INFECT DIS, V44, P1401

[4] Characterization of epitopes for virus-neutralizing monoclonal antibodies to Ross River virus E2 using phage-displayed random peptide libraries [J].

Davies, JM ;

Cai, YP ;

Weir, RC ;

Rowley, MJ .

VIROLOGY, 2000, 275 (01) :67-76

[5] Molecular diagnosis and analysis of Chikungunya virus [J].

Edwards, Carolyn J. ;

Welch, Stephen R. ;

Chamberlain, John ;

Hewson, Roger ;

Tolley, Howard ;

Cane, Patricia A. ;

Lloyd, Graham .

JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (04) :271-275

[6] Chikungunya fever resurgence and global warming [J].

Epstein, Paul R. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 76 (03) :403-404

[7] DETECTION OF ALPHAVIRUSES IN A GENUS-SPECIFIC ANTIGEN CAPTURE ENZYME-IMMUNOASSAY USING MONOCLONAL-ANTIBODIES [J].

GREISERWILKE, IM ;

MOENNIG, V ;

KAADEN, OR ;

SHOPE, RE .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (01) :131-137

[8]

HADDOW A. J., 1960, TRANS ROY SOC TROP MED AND HYG, V54, P517, DOI 10.1016/0035-9203(60)90025-0

[9]

Johnston R.E., 1996, Virology, 3rd Edition, P843

[10] Emergence of epidemic O'nyong-nyong fever in Uganda after a 35-year absence: Genetic characterization of the virus [J].

Lanciotti, RS ;

Ludwig, ML ;

Rwaguma, EB ;

Lutwama, JJ ;

Kram, TM ;

Karabatsos, N ;

Cropp, BC ;

Miller, BR .

VIROLOGY, 1998, 252 (01) :258-268

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