MALDI-TOF-MS analysis of protein and DNA

被引：80

作者：

Bonk, T ^{[1
]}

Humeny, A ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Inst Biochem, D-91054 Erlangen, Germany

来源：

NEUROSCIENTIST | 2001年 / 7卷 / 01期

关键词：

MALDI; mass spectrometry; neurochemistry; genetic analysis; protein analysis;

D O I：

10.1177/107385840100700104

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a powerful and widespread analytical tool in all fields of life science. The wide mass range (1-300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectrophoresis, MALDI-TOF-MS is particularly suitable for the identification of protein spots via mass fingerprint or microsequencing. Furthermore, the method allows a detailed analysis of posttranslational protein modifications. Recently, the method was also successfully applied to DNA sequencing as well as screening for mutations. Thus, high-throughput genotyping of single nucleotide polymorphisms has the potential to become a routine method for both laboratory and clinical applications.

引用

页码：6 / 12

页数：7

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