Sequence specificity of the hammerhead ribozyme revisited; the NHH rule

被引：58

作者：

Kore, AR ^{[1
]}

Vaish, NK ^{[1
]}

Kutzke, U ^{[1
]}

Eckstein, F ^{[1
]}

机构：

[1] Max Planck Inst Expt Med, D-37075 Gottingen, Germany

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 18期

关键词：

D O I：

10.1093/nar/26.18.4116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence specificity of hammerhead ribozyme cleavage has been re-evaluated with respect to the NUH rule. Contrary to previous reports it was found that substrates with GAC triplets were also cleaved. This was established in three different sequence contexts. The rate of cleavage under single turnover conditions was between 3 and 7% that of cleavage 3' of GUC. Specificity of cleavage of substrates containing a central A in the cleavable triplet can be described as NAH, where N can be any nucleotide and H any nucleotide but G. As cleavage 3' of NCH triplets has recently been described, the NUH rule can be reformulated to NHH.

引用

页码：4116 / 4120

页数：5

共 20 条

[1] A kinetic and thermodynamic analysis of cleavage site mutations in the hammerhead ribozyme [J].