Specific cleavage of γ catenin by caspases during apoptosis

被引:27
作者
Schmeiser, K [1 ]
Hammond, EM [1 ]
Roberts, S [1 ]
Grand, RJA [1 ]
机构
[1] Univ Birmingham, CRC, Inst Canc Studies, Birmingham B15 2TA, W Midlands, England
关键词
gamma catenin; plakoglobin; alpha catenin; apoptosis; caspase; cytoskeleton;
D O I
10.1016/S0014-5793(98)00850-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Caspase-mediated proteolysis of cytoskeletal proteins during apoptosis appears to be commonplace. Enlarging on previous studies me have shown here that gamma catenin, like beta catenin, was degraded during cisplatin-induced apoptosis, initially giving a major product of 75 kDa, This truncated protein could be coimmunoprecipitated with alpha catenin, Addition of caspase inhibitors to cells in the presence of cisplatin appreciably reduced the proteolysis of gamma catenin as well as the level of apoptosis, Only limited degradation of alpha catenin was observed even at very late times when over 90% of cells in the culture mere apoptotic, Immunohistochemical staining showed that during apoptosis there mas a relocation of alpha, beta and gamma catenin from the periphery of the cell to the cytoplasm, at the same time as other morphological changes commonly associated with apoptosis occurred. Interestingly, the changes in localisation of the catenins preceded proteolysis by several hours, In the presence of cisplatin and caspase inhibitor no change in distribution of catenins was observed, suggesting that re-localisation requires caspase activity but not necessarily directed against beta and gamma catenins, (C) 1998 Federation of European Biochemical Societies.
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收藏
页码:51 / 57
页数:7
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