α-Amylase production in recombinant Aspergillus oryzae during fed-batch and continuous cultivations

被引：28

作者：

Spohr, A ^{[1
]}

Carlsen, M ^{[1
]}

Nielsen, J ^{[1
]}

Villadsen, J ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Biotechnol, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1998年 / 86卷 / 01期

关键词：

Aspergillus oryzae; alpha-amylase; fed-batch fermentations; enzyme production;

D O I：

10.1016/S0922-338X(98)80033-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three a-amylase producing strains of Aspergillus oryzae used for recombinant protein production have been studied with respect to growth and protein production in fed-batch and continuous cultures. The three strains are (i) a wild-type, (ii) a transformant strain of the wild-type strain containing additional copies of the a-amylase gene and (iii) a morphological mutant of the transformant strain. It is found that production of a-amylase is a growth related process. Comparison of the three strains with respect to morphology and protein production reveals that changes in the morphology of the fungus influence the a-amylase production during submerged growth.

引用

页码：49 / 56

页数：8

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