Annexin VII:: an astroglial protein exhibiting a Ca2+-dependent subcellular distribution

被引:16
作者
Clemen, CS
Herr, C
Lie, AA
Noegel, AA
Schröder, R
机构
[1] Univ Cologne, Fac Med, Inst Biochem, D-50931 Cologne, Germany
[2] Univ Bonn, Fac Med, Dept Epileptol, D-53105 Bonn, Germany
[3] Univ Bonn, Fac Med, Dept Neurol, D-53105 Bonn, Germany
关键词
annexin VII; astroglia; Ca2+-and phospholipid-binding protein; C6 cell line; GFP annexin VII fusion; human brain; secretion;
D O I
10.1097/00001756-200105080-00018
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A fundamental issue in neuronal and glial cells is how intracellular rises in Ca2+ are coupled to signaling cascades and changes in subcellular morphology. We studied the expression and localization of annexin VII (synexin), a Ca2+-IGTP-dependent membrane fusion protein, in the human CNS. Here, we demonstrate the presence of two annexin VII isoforms (47 and 51 kDa) in human brain tissue as well as its exclusive expression in astroglial cells, An in vitro study of astrocyte-derived Cb rat glioblastoma cells expressing a GFP tagged annexin VII fusion protein demonstrates a sequential redistribution of the fusion protein in response to rising intracellular Ca2+ concentrations. Our findings indicate a role of annexin VII in the regulation of intracellular Ca2+-dependent processes in astroglial cells. NeuroReport 12:1139-1144 (C) 2001 Lippincott Williams & Wilkins.
引用
收藏
页码:1139 / 1144
页数:6
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