Effect of hyperthermia on invasion ability and TGF-β1 expression of breast carcinoma MCF-7 cells

被引：53

作者：

Xie, Xiaoxue ^{[1
,2
]}

Shao, Xunfan ^{[3
]}

Gao, Fuping ^{[1
,2
]}

Jin, Hekun ^{[1
,2
,4
]}

Zhou, Jumei ^{[1
,2
,4
]}

Du, Lehui ^{[1
,2
]}

Zhang, Yingying ^{[1
,2
]}

Ouyang, Weiwei ^{[1
,2
]}

Wang, Xiaowen ^{[1
,2
]}

Zhao, Lingyun ^{[1
,2
]}

Zhang, Xiaodong ^{[1
,2
]}

Tang, Jintian ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Inst Med Phys & Engn, Dept Engn Phys, Beijing 100084, Peoples R China

[2] Tsinghua Univ, Key Lab Particle & Radiat Imaging, Minist Educ, Beijing 100084, Peoples R China

[3] Guangzhou Med Univ, Affiliated Tumor Hosp, Guangzhou 510095, Guangdong, Peoples R China

[4] Hunan Prov Tumor Hosp, Dept Radiotherapy, Changsha, Hunan, Peoples R China

来源：

ONCOLOGY REPORTS | 2011年 / 25卷 / 06期

基金：

中国国家自然科学基金;

关键词：

hyperthermia; MCF-7; cells; cancer; invasion; 1-MATRIX METALLOPROTEINASE PRODUCTION; ENDOTHELIAL GROWTH-FACTOR; TGF-BETA; TRANSFORMING GROWTH-FACTOR-BETA-1; TUMOR PROGRESSION; METASTASIS; PROGELATINASE; FIBROBLASTS; SUPPRESSION; PROGNOSIS;

D O I：

10.3892/or.2011.1240

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-beta(1) expression. MCF-7 cells were heated at 43, 45, 47 and 37 degrees C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-beta(1) (TGF-beta(1)) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-beta(1) mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-beta(1), EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.

引用

页码：1573 / 1579

页数：7

共 30 条

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