Re-evaluation of the primary structure of Ralstonia eutropha phasin and implications for polyhydroxyalkanoic acid granule binding

被引：37

作者：

Hanley, SZ ^{[1
]}

Pappin, DJC

Rahman, D

White, AJ

Elborough, KM

Slabas, AR

机构：

[1] Univ Durham, Dept Biol Sci, Durham DH1 3LE, England

[2] Imperial Canc Res Fund, London WC2A 3PX, England

来源：

FEBS LETTERS | 1999年 / 447卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Alcaligenes eutrophus; Ralstonia eutropha; polyhydroxyalkanoic acid granule; phasin; biodegradable polyester;

D O I：

10.1016/S0014-5793(99)00235-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence analysis of several cDNAs encoding the phasin protein of Ralstonia eutropha indicated that the carboxyl terminus of the resulting derived protein sequence is different from that reported previously. This mas confirmed by: (1) sequencing of the genomic DNA; (2) SDS-PAGE and peptide analysis of wild-type and recombinant phasin; and (3) mass spectrometry of wild-type phasin protein, The results have implications for the model proposed for the binding of this protein to polyhydroxyalkanoic acid granules in the bacterium. (C) 1999 Federation of European Biochemical Societies.

引用

页码：99 / 105

页数：7

共 27 条

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