Electrochemical immunoassay for CA 125 based on cellulose acetate stabilized antigen/colloidal gold nanoparticles membrane

被引：77

作者：

Wu, LN ^{[1
]}

Chen, J ^{[1
]}

Du, D ^{[1
]}

Ju, HX ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Educ Minist China, Key lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

来源：

ELECTROCHIMICA ACTA | 2006年 / 51卷 / 07期

基金：

中国国家自然科学基金;

关键词：

immunosensor; electrochemical sensors; biosensors; CA; 125; cellulose acetate; colloidal gold nanoparticles; immunoassay;

D O I：

10.1016/j.electacta.2005.06.011

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A novel separation-free electrochemical immunosensor for carcinoma antigen- 125 (CA 125) was proposed based on the immobilization of CA125 antigen on colloidal gold nanoparticles that was stabilized with cellulose acetate membrane on a glassy carbon electrode. A competitive immunoassay format was employed to detect CA125 antigen with horseradish peroxidase (HRP) labeled CA125 antibody as tracer, o-phenylenediamine and hydrogen peroxide as enzyme substrates. After the immunosensor was incubated with a mixture of HRP labeled CA125 antibody and CA125 sample at 35 degrees C for 50 min, the amperometric response decreased with an increasing CA125 concentration in the sample solution. The decreased percentage of the electrocatalytic current was proportional to CA125 concentration ranging from 0 to 30 U ml(-1) with a detection limit of 1.73 U ml(-1) (S/N = 3). The proposed immunosensor showed good stability, acceptable accuracy, and would be applicable to clinical immunoassay of CA125. (C) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1208 / 1214

页数：7

共 43 条

[1] New variants of enzyme immunoassay of antibodies to DNA [J].