Analyzing CRISPR genome-editing experiments with CRISPResso

被引：324

作者：

Pinello, Luca ^{[1
,2
]}

Canver, Matthew C. ^{[3
,4
,5
,6
]}

Hoban, Megan D. ^{[7
]}

Orkin, Stuart H. ^{[3
,4
,5
,6
,8
]}

Kohn, Donald B. ^{[7
]}

Bauer, Daniel E. ^{[3
,4
,5
,6
]}

Yuan, Guo-Cheng ^{[1
,2
]}

机构：

[1] Dana Farber Canc Inst, Dept Biostat & Computat Biol, Boston, MA 02115 USA

[2] Harvard TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

[3] Boston Childrens Hosp, Div Hematol Oncol, Boston, MA USA

[4] Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA

[5] Harvard Univ, Harvard Med Sch, Dept Pediat, Boston, MA 02115 USA

[6] Harvard Univ, Harvard Stem Cell Inst, Boston, MA 02115 USA

[7] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA USA

[8] Howard Hughes Med Inst, Boston, MA 02115 USA

来源：

NATURE BIOTECHNOLOGY | 2016年 / 34卷 / 07期

关键词：

CAS9; SPECIFICITY; NUCLEASES; SYSTEM; CELLS;

D O I：

10.1038/nbt.3583

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

引用

页码：695 / 697

页数：4

共 9 条

[1] Characterization of Genomic Deletion Efficiency Mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 Nuclease System in Mammalian Cells [J].

Canver, Matthew C. ;

Bauer, Daniel E. ;

Dass, Abhishek ;

Yien, Yvette Y. ;

Chung, Jacky ;

Masuda, Takeshi ;

Maeda, Takahiro ;

Paw, Barry H. ;

Orkin, Stuart H. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (31) :21312-21324

[2] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

[3] High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells [J].

Fu, Yanfang ;

Foden, Jennifer A. ;

Khayter, Cyd ;

Maeder, Morgan L. ;

Reyon, Deepak ;

Joung, J. Keith ;

Sander, Jeffry D. .

NATURE BIOTECHNOLOGY, 2013, 31 (09) :822-+

[4] Genome editing assessment using CRISPR Genome Analyzer (CRISPR-GA) [J].

Gueell, Marc ;

Yang, Luhan ;

Church, George M. .

BIOINFORMATICS, 2014, 30 (20) :2968-2970

[5] DNA targeting specificity of RNA-guided Cas9 nucleases [J].

Hsu, Patrick D. ;

Scott, David A. ;

Weinstein, Joshua A. ;

Ran, F. Ann ;

Konermann, Silvana ;

Agarwala, Vineeta ;

Li, Yinqing ;

Fine, Eli J. ;

Wu, Xuebing ;

Shalem, Ophir ;

Cradick, Thomas J. ;

Marraffini, Luciano A. ;

Bao, Gang ;

Zhang, Feng .

NATURE BIOTECHNOLOGY, 2013, 31 (09) :827-+

[6] RNA-Guided Human Genome Engineering via Cas9 [J].

Mali, Prashant ;

Yang, Luhan ;

Esvelt, Kevin M. ;

Aach, John ;

Guell, Marc ;

DiCarlo, James E. ;

Norville, Julie E. ;

Church, George M. .

SCIENCE, 2013, 339 (6121) :823-826

[7] Genome engineering using the CRISPR-Cas9 system [J].

Ran, F. Ann ;

Hsu, Patrick D. ;

Wright, Jason ;

Agarwala, Vineeta ;

Scott, David A. ;

Zhang, Feng .

NATURE PROTOCOLS, 2013, 8 (11) :2281-2308

[8] Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity [J].

Ran, F. Ann ;

Hsu, Patrick D. ;

Lin, Chie-Yu ;

Gootenberg, Jonathan S. ;

Konermann, Silvana ;

Trevino, Alexandro E. ;

Scott, David A. ;

Inoue, Azusa ;

Matoba, Shogo ;

Zhang, Yi ;

Zhang, Feng .

CELL, 2013, 154 (06) :1380-1389

[9] CRISPR-Cas systems for editing, regulating and targeting genomes [J].

Sander, Jeffry D. ;

Joung, J. Keith .

NATURE BIOTECHNOLOGY, 2014, 32 (04) :347-355

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